Lei Jin, PhD, MSc

Lei Jin, PhD, MSc
Assistant Professor of Medicine

Email: Lei.Jin@medicine.ufl.edu
Phone: 352.294.8495

 

Degree/Program

Institution

Field/Specialty

BSc Fudan University, China Biophysics
MSc Fudan University, China Human Genetics
PhD Tufts University Genetics
Postdoctoral Fellow National Jewish Health/University of Colorado Denver Immunology

Vaccines are one of the greatest achievements of biomedical science. Most vaccines are injected. There is a growing interest in developing and improving vaccines that can be inhaled. This delivers the vaccine directly to the mucosal surfaces that line the nose and lungs, which is a more effective way to produce immunity against respiratory pathogens such as pneumococcus and influenza. As these mucosal vaccines are also relatively cheap and easy to apply, they would also be suitable for use in developing countries and during emergencies. Developing safe and effective mucosal vaccine adjuvants, which help to boost targeted immune response, is key to the development of mucosal vaccines. Currently, there is no approved mucosal vaccine adjuvant in U.S.

Cyclic dinucleotides (CDNs) are promising mucosal vaccine adjuvant candidates. They elicit potent and balanced humoral and cellular immune response protecting animals from virus and bacterial infection. CDNs include cyclic di-AMP (CDA), cyclic di-GMP (CDG) and the newly discovered 2’5’-3’5’-cyclic GMP-AMP (2’3’-cGAMP). STING (stimulator of interferon genes) is the mammalian receptor for CDNs. We first showed that the mucosal adjuvant CDNs enhance antigen uptake and process in animals and directly activates dendritic cells (DC) in vivo. We further showed that STING-dependent TNFα production is critical for the mucosal adjuvant activity of CDNs in vivo.

Our goal is to i) decipher the in vivo cellular and molecular mechanism of action of CDN; ii) advance CDN as safe and efficacious mucosal vaccine adjuvants in human. The approaches we are using including knockout, knockin mice, conditional knockout, and conditional WT mice. We are also endeavoring to expand our research in human samples and cells.

Other research interests in the lab include i) Understanding the effects of common human STING variations on licensed pneumococcal vaccines Pneumovax®23 and Prevnar13® effectiveness in vivo; ii) Understanding the role of STING in host defense against pneumococcal  infections in vivo.

Professional Societies

  • American Association of Immunologists
  • International Cytokine and Interferon Society

Honor/Award

  • 2012 – Marie Curie Fellow – European Commission
  • 2015 – The Gary and Janis Grover Young Scientist Award- Albany Medical College

Grant Support

  • Ongoing Research Support
    • NIH-5R01AI110606: Mechanisms of STING-Mediated Mucosal Vaccine Adjuvant Activity of Cyclic di-GMP –  2014 ~2019
    • NIH-1R21AI125999: Impact of Human STING Variants on Pneumococcal Vaccine Effectiveness2016~2018
  • Completed Research Support
    • NIH-R56AI110606: Mechanisms of STING-Mediated Mucosal Vaccine Adjuvant Activity of Cyclic di-GMP –  July 2014 ~ Nov 2014
    • NIH-R21AI099346 (Subcontract): Mouse Modeling of a Human STING Gene Variant for Infectious Disease2012 ~ 2015

Publications